The Flow NanoAnalyzer paves a way for the mutiparameter analysis of the nanoparticles from 7 to 1000 nm with its unprecedented sentivity in scatterring and fluorescence detection. The Flow NanoAnalyzer delivers high-resolution data at the single-particle level to dicipher the subpopulations and compositions of various nanoparticles, such as extracellular vesicles, LNPs and virial vectors.