NanoFCM Deciphering Bio-Nanoparticles

Sizing,Concentration & Phenotyping

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  • Laser (blue, green, red)
  • One side-scatter channel
  • Two fluorescence channels
  • up to 12,000 particles/min
  • Lower detection limit 7 nm (gold nanoparticles) to 40 nm (exosomes, viruses)
  • High detection limit (1 micron)
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Comprehensive LNP Characterization from Cargo to Target Modification Using Nano-Flow Cytometry  :

Optimizing RNA encapsulation is vital for formulation development and maximizing delivery to target cells to ensure the success of therapeutics and vaccines. However assessing the efficiency of the optimization process remains challenging. One solution is single particle analyses using technologies like nano-flow cytometry. This technology makes it possible to simultaneously size and count lipid nanoparticles (LNPs) whilst identifying the presence of cargo or surface modifications to determine efficacy of the nanoparticle loading or functionalization.

Date : 

Wednesday, July 10, 2024

Time : 

8:00 am PT | 11:00 am ET | 17:00 CET

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EXTRACELLULAR VESICLES

THE ORIGIN AND PHENOTYPING OF EXTRACELLULAR VESICLES

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VACCINE

COMPREHENSIVE MEASUREMENT SUITE FOR NANOSIZED DRUG PRODUCTS

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BACTERIA DETECTION

TRULY QUANTITATIVE MEASUREMENT OF TOTAL BACTERIAL TITRE

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VIRUSES/VIRAL VACCINE

INTEGRATE INTO THE GENOMES OF THE HOST CELLS

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OTHERS

COMPREHENSIVE MEASUREMENT SUITE FOR NANOPARTICLES AND INDIVIDUAL MITOCHONDRIA

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NANOMEDICINE

COMPREHENSIVE MEASUREMENT SUITE FOR NANOSIZED DRUG PRODUCTS

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EXTRACELLULAR VESICLES

THE ORIGIN AND PHENOTYPING OF EXTRACELLULAR VESICLES

DETAILS
team_img

VACCINE

COMPREHENSIVE MEASUREMENT SUITE FOR NANOSIZED DRUG PRODUCTS

DETAILS
team_img

BACTERIA DETECTION

TRULY QUANTITATIVE MEASUREMENT OF TOTAL BACTERIAL TITRE

DETAILS
team_img

VIRUSES/VIRAL VACCINE

INTEGRATE INTO THE GENOMES OF THE HOST CELLS

DETAILS
team_img

OTHERS

COMPREHENSIVE MEASUREMENT SUITE FOR NANOPARTICLES AND INDIVIDUAL MITOCHONDRIA

DETAILS
team_img

NANOMEDICINE

COMPREHENSIVE MEASUREMENT SUITE FOR NANOSIZED DRUG PRODUCTS

DETAILS
team_img

EXTRACELLULAR VESICLES

THE ORIGIN AND PHENOTYPING OF EXTRACELLULAR VESICLES

team_img

VACCINE

COMPREHENSIVE MEASUREMENT SUITE FOR NANOSIZED DRUG PRODUCTS

team_img

BACTERIA DETECTION

TRULY QUANTITATIVE MEASUREMENT OF TOTAL BACTERIAL TITRE

team_img

VIRUSES/VIRAL VACCINE

INTEGRATE INTO THE GENOMES OF THE HOST CELLS

team_img

OTHERS

COMPREHENSIVE MEASUREMENT SUITE FOR NANOPARTICLES AND INDIVIDUAL MITOCHONDRIA

team_img

NANOMEDICINE

COMPREHENSIVE MEASUREMENT SUITE FOR NANOSIZED DRUG PRODUCTS

Size Distribution

High-resolution size distribution comparable to TEM

DETAILS

Particle Concentration

Measure the absolute concentration of both total particles and subpopulations

DETAILS

Multiparameter

Quantify sub-populations with up to 2 markers simultaneously

DETAILS

Phenotyping

Based on single -molecule fluorescence detection, Phenotype quantify direct from marker labeling intensity

DETAILS

Size Distribution

Particle Concentration

Multiparameter

Phenotyping

For many years scientists yearned for the possibility of performing flow cytometry to analyse small bio-nanoparticles that are too small to be measured by conventional and high sensitivity instruments. These entities, extracellular vesicles, gene therapy vectors, viruses and drug delivery particles, are promised to become the next generation of therapeutics, but they have been hard to handle and characterise due to their small size and biological or chemical heterogeneity. There is therefore a strong case for bringing flow cytometry capability to the sub-200nm scale.

NanoFCM has developed the NanoAnalyzer platform that now enables true flow-cytometry measurement at the sub-micron scale, and down to particle sizes unreachable by any other flow cytometers (10-40nm depending on the nature of the substrate). Nano-flow cytometry, the technology that underpins the NanoAnalyzer, removes bias and uncertainty stemming from the use of fluorescence signal triggering by using its highly sensitive side-scatter channel to trigger particle events. The single-particle nature of the measurement prevents uncontrolled swarming events, reinforcing data integrity. High resolution of both scatter and fluorescence channels allows the assessment of subpopulations, based on size or on bio-chemical properties.

Nano-flow cytometry’s ability to measure simultaneously a (bio)-nanoparticle population for size, size distribution and bio-chemical properties on a single instrument dramatically improves data quality and throughput compared to the traditional, multiple-techniques approach involving particle characterisation and counting (DLS, NTA, RPS), combined with chemical and biological function assessment (ELISA, Western Blot, Flow Cytometry, PCR). Quantitative measurement of the active and contaminant particles in a single preparation opens up the possibility of characterisation-based nanomedicine regulatory approval, and allows the conduct of large-scale clinical studies. From the research laboratory to the quality control department, NanoFCM delivers comprehensive bio-nano analysis.