High Performance

Ultra-Sensitive and High Resolution for both scatter and fluorescence.

Author: Nanofcm     Date: March 4, 2021

SIDE SCATTER

Nano-flow Cytometry is super-sensitive, which allows label free analysis of single silica and gold nanoparticles down to 24 nm and 7 nm, respectively. For viruses, it is able to detect the signals from single MS2 virus (27 nm).Nano-flow cytometry shows a resolution comparable to that of TEM and the throughput of flow cytometric analysis.

FLUORESCENCE

The fluorescent signals from single R-phycoerythrin molecules can be acquired individually by nano-flow cytometry, which means the NanoFCM is able to study EV proteins as low as ONE copy.

HIGH-SPEED & HIGH-THOUGHPUT

Hundreds of nanoparticles can be detected one by one per second, and a highly representative statistical distribution of particle properties can be obtained in 1 minute.

No Swarm Detection

Real time, single particle measurement

Author: Nanofcm     Date: March 4, 2021

The real-time burst traces are monitored/collected to ensure the particles are detected individually. The state of the probe volume is monitored in real time.

Label Free

Direct size and concentration measurement without staining

Author: Nanofcm     Date: March 4, 2021

Measure the intrinsic side scatter signal directly from the particles, there is no need to label the particles to get the size and concentration information. The physical properties are acquired without manipulating samples.

Flexible

No proprietary reagents, choose from a vast array of commercially available markers

Author: Nanofcm     Date: March 4, 2021

Users can choose their proteins of interest (universal antibodies, aptamers) and conjugated dyes freely, the illuminating time is in the range of millisecond, there is no need to worry about the photobleaching.

Small sample volume requirements：Sample injection speed down to ~ nL/min level, basically no sample consumption, and the sample can be recycled.

Size Distribution

High-resolution size distribution comparable to TEM

Author: Nanofcm     Date: March 4, 2021

Compared with polystyrene beads (RI=1.59), in nFCM system monodisperse silica nanoparticles (RI=1.46) are employed as the reference to calibrate the size of EVs.

The resolution of NanoFCM is comparable to TEM. Median, mean, and standard deviation value of a sample can be read from the software, parameter of gated population within a sample is also available.

Particle Concentration

Measure the absolute concentration of both total particles and subpopulations

Author: Nanofcm     Date: March 4, 2021

In NanoFCM system, the detection efficiency is 100%, the particle concentration is determined via single particle enumeration. The particle counts collected within given time, particle concentration of whole population as well as subpopulation can be determined， e.g. the ratio of positive. The biochemical function of a subgroup is linked with its size distribution.

Multiparameter

Quantify sub-populations with up to 2 markers simultaneously

Author: Nanofcm     Date: March 4, 2021

Multiple parameters, including various surface proteins, nucleic acids, lipids and so on can be achieved within a sample simultaneously.

Phenotyping

Based on single -molecule fluorescence detection, Phenotype quantify direct from marker labeling intensity.

Author: Nanofcm     Date: March 4, 2021

After labeling with fluorescent probes, the fluorescence signals of EVs can be detected concurrently with side scatter. By offering quantitatively analysis of single EVs within a sample, the copy number of proteins, payload of engineered EVs can be evaluated individually.