Compared with polystyrene beads (RI=1.59), in nFCM system monodisperse silica nanoparticles (RI=1.46) are employed as the reference to calibrate the size of EVs.
The resolution of NanoFCM is comparable to TEM. Median, mean, and standard deviation value of a sample can be read from the software, parameter of gated population within a sample is also available.
In NanoFCM system, the detection efficiency is 100%, the particle concentration is determined via single particle enumeration. The particle counts collected within given time, particle concentration of whole population as well as subpopulation can be determined, e.g. the ratio of positive. The biochemical function of a subgroup is linked with its size distribution.