Author: Nanofcm     Date: March 4, 2021

Extracellular Vesicles (Exosomes)

Measuring particle concentration of multimodal synthetic reference materials and extracellular vesicles with orthogonal techniques: Who is up to the challenge?
Vogel R, Savage J, Muzard J, et al.
Journal of Extracellular Vesicles, 2021

Comprehensive evaluation of methods for small extracellular vesicles separation from human plasma, urine and cell culture medium.
Dong L, Zieren R C, et al.
Journal of Extracellular Vesicles, 2021

Plasma extracellular vesicles detected by Single Molecule array technology as a liquid biopsy for colorectal cancer.
Wei P, Wu F, Kang B, et al.
Journal of Extracellular Vesicles, 2020

The future of Extracellular Vesicles as Theranostics– an ISEV meeting report.
Soekmadji C, Li B, Huang Y, et al.
Journal of Extracellular Vesicles, 2020

Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles.
Jiang W, Ma P, Deng L, et al.
Journal of Extracellular Vesicles, 2020

Quality and efficiency assessment of six extracellular vesicle isolation methods by nano-flow cytometry.
Tian Y, Gong M, Hu Y, et al.
Journal of Extracellular Vesicles, 2020

A Versatile Platform for Generating Engineered Extracellular Vesicles with Defined Therapeutic Properties.
Dooley K, McConnell, R. E. et al.
Molecular Therapy, 2021

Extracellular Vesicle Delivery of TRAIL Eradicates Resistant Tumor Growth in Combination with CDK Inhibition by Dinaciclib.
Ke C, Hou H., Li J et al.
Cancers, 2020

Extracellular vesicle isolation from human renal cancer tissue.
Zieren R C, Dong L, Pierorazio P M, et al.
Medical Oncology, 2020

Plasma Extracellular Vesicle Long RNA Profiling Identifies a Diagnostic Signature for the Detection of Pancreatic Ductal Adenocarcinoma.
Yu S, Li Y, and Liao Z, et al.
Gut., 2019

Extracellular Vesicle-Based Coatings Enhance Bioactivity of Titanium Implants—SurfEV
Pansani, T N, Phan T H, Chrzanowski W, et al.
Nanomaterials, 2021

New Multiscale Characterization Methodology for Effective Determination of Isolation–Structure–Function Relationship of Extracellular Vesicles
Phan T H, Divakarla S K, Chrzanowski W, et al.
Frontiers in Bioengineering and Biotechnology, 2021

Radiation-induced small extracellular vesicles as “carriages” promote tumor antigen release and trigger antitumor immunity.
Lin W, Xu Y, Chen X, et al.
Theranostics, 2020

Relationships of Alzheimer’s disease and apolipoprotein E genotypes with small RNA and protein cargo of brain tissue extracellular vesicles.
Huang Y, Driedonks T A, Cheng L, et al.
medRxiv, 2020

Extracellular vesicles containing host restriction factor IFITM3 inhibited Zika virus infection of fetuses in pregnant mice through trans-placenta delivery.
Zou X, Yuan M, Zhang T,  et al.
Molecular Therapy, 2020

Characterization of extracellular vesicles and artificial nanoparticles with four orthogonal single-particle analysis platforms.
Mallick E R., Arab, Huang al.
bioRxiv, 2021

Small Extracellular Vesicles From Human Adipose-derived Stem Cells: A Potential Promoter of Keeping Fat Graft Survival.
Chen A, Tang S, He J, et al
Stem Cell Research & Therapy 2020

Therapeutic mechanism of human neural stem cell-derived extracellular vesicles against hypoxia-reperfusion injury in vitro.
Liu Q, Tan Y, Qu T, et al.
Life Sciences, 2020

ESC‐sEVs Rejuvenate Senescent Hippocampal NSCs by Activating Lysosomes to Improve Cognitive Dysfunction in Vascular Dementia.
Hu G, Xia Y, Zhang J, et al.
Advanced Science, 2020

Increased Interleukin-6 Levels in Neuron-Derived Plasma Small Extracellular Vesicles of Subarachnoid Haemorrhage Patients.
Lai N, Yao Y, Yu T, et al.
Research Square, 2020

Small extracellular vesicles secreted by human iPSC-derived MSC enhance angiogenesis through inhibiting STAT3-dependent autophagy in ischemic stroke.
Xia Y, Ling X, Hu G, et al.
Stem Cell Research & Therapy, 2020

The immunomodulatory signature of extracellular vesicles from Cardiosphere-Derived Cells: a proteomic and miRNA profiling.
López E, Marinaro F, de Pedro, et al.
Frontiers in Cell and Developmental Biology, 2020

Placenta Stem/Stromal Cell–Derived Extracellular Vesicles for Potential Use in Lung Repair.
Kim S Y, Joglekar M V, Hardikar A A, et al.
Proteomics, 2019

Synthetic Antigen Presenting cells reveal the diversity and functional specialisation of extracellular vesicles composing the fourth signal of  T cell immunological synapses
L Dustin M, Cespedes P, et al.
bioRxiv, 2021

Technologies and Standardization in Research on Extracellular Vesicles.
Gandham S, Su X, Wood J, et al.
Trends in Biotechnology, 2020

Separation of extracellular vesicles by DNA-directed immunocapturing followed by enzymatic release.
Brambilla D, Sola L, Chiodi, et al.
ChemRixv , 2020

Protein Profiling and Sizing of Extracellular Vesicles from Colorectal Cancer Patients via Flow Cytometry.
Tian Y, Ma L, Gong M, et al.
ACS Nano, 2018

Small extracellular vesicles derived from embryonic stem cells restore ovarian function of premature ovarian failure through PI3K/AKT signaling pathway.
Liu M, Qiu Y, Xue Z, et al.
Stem Cell Research & Therapy, 2020

Programmable Modulation for Extracellular Vesicles. 
Wany Y, Melvin R, Bemis L, Worrell G, Wang H.
bioRxiv, 2019

Extracellular Vesicles Long RNA Sequencing Reveals Abundant mRNA, circRNA, and lncRNA in Human Blood as Potential Biomarkers for Cancer Diagnosis.
Li Y, Zhao J, and Huang S et al.
Clinical Chemistry, 2019

Clinical significance of circulating exosomal PD-L1 and soluble PD-L1 in extranodal NK/T-cell lymphoma, nasal-type.
Li J. W, Wei P, Guo, et al.
American journal of cancer research, 2020

Nonmuscle Myosin Heavy Chain ⅡA-Mediated Exosome Release via Regulation of the Rho-Associated Kinase 1/Myosin Light Chains/Actin Pathway.
Lv, Y., Chen, J., Hu, J., et al.
Frontiers in Pharmacology, 2020

Exosomal CA125 as A Promising Biomarker for Ovarian Cancer Diagnosis.
Chen Z, Liang Q, Zeng H, et al.
Journal of Cancer, 2020

Thermosensitive Exosome–Liposome Hybrid Nanoparticle‐Mediated Chemoimmunotherapy for Improved Treatment of Metastatic Peritoneal Cancer.
Lv Q, Cheng L, Lu Y, et al.
Advanced Science, 2020

Exosomal lncRNA RAMP2-AS1 Derived from Chondrosarcoma Cells Promotes Angiogenesis Through miR-2355-5p/VEGFR2 Axis.
Cheng C, Zhang Z, Cheng F, et al.
OncoTargets and therapy, 2020

Serum Exosomal MicroRNA-21, MicroRNA-126, and PTEN Are Novel Biomarkers for Diagnosis of Acute Coronary Syndrome.
Ling H, Guo Z, Shi Y, et al.
Frontiers in Physiology, 2020

Clinical Significance of Circulating Exosomal PD-L1 and Soluble PD-L1 in Nasal-type NK/T-cell Lymphoma Patients, Receiving VIPD Containing Chemotherapy.
Jiwei Li, Ping Wei, Ye Guo, et al.
Research Square, 2020

Extracellular Vesicles Derived from ODN-stimulated Macrophages Transfer and Activate Cdc42 in Recipient Cells and Thereby Increase Cellular Permissiveness to EV Uptake.
Zhang Y, Jin X, and Liang J et al.
Science Advances, 2019

Exosomes Derived from Umbilical Cord Mesenchymal Stem Cells Alleviate Mifepristone-Induced Human Endometrial Stromal Cell Injury.
Wang J, Hu R, Xing Q, et al.
Stem Cells International, 2020

PTH Derivative promotes wound healing via synergistic multicellular stimulating and exosomal activities.
Shen Y F, Huang J H, Wang K.Y, et al.
Cell Communication and Signaling, 2020

Articular chondrocyte derived exosomes promote cartilage differentiation of human umbilical cord mesenchymal stem cells by activation of autophagy.
Ma K, Zhu B, Wang Z, et al.
Journal of Nanobiotechnology, 2020

Mesenchymal stem cell-derived exosomes protect beta cells against hypoxia-induced apoptosis via miR-21 by alleviating ER stress and inhibiting p38 MAPK phosphorylation.
Chen J, Chen J, Cheng Y, et al.
Stem cell research & therapy, 2020

Seminal plasma exosomes evoke calcium signal via CatSper channel to regulate human sperm function.
Zhang X, Song D, Zhou W, et al.
bioRxiv, 2020

Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2/TLR4 Signaling Pathway.
Yang C, Sun S, Zhang Q, et al.
Stem Cells International, 2020

Exosomes isolated from the plasma of remote ischemic conditioning rats improved cardiac function and angiogenesis after myocardial infarction through targeting Hsp70.
Chen Q, Huang M, Wu J, et al.
Aging (Albany NY), 2020

RNA sequencing of exosomes revealed differentially expressed long noncoding RNAs in early-stage esophageal squamous cell carcinoma and benign esophagitis.
Tian L, Yang L, Zheng W, et al.
Epigenomics, 2020

The expression of small RNAs in exosomes of follicular fluid altered in human polycystic ovarian syndrome.
Hu J, Tang T, Zeng Z, et al.
PeerJ, 2020

Saliva Exosomes-Derived UBE2O Promotes Angiogenesis in Cutaneous Wounds By Targeting SMAD6.
Mi B, Chen L, Xiong Y, et al.
Journal of Nanobiotechnology, 2020

Exosomes from human urine‐derived stem cells enhanced neurogenesis via miR‐26a/HDAC6 axis after ischaemic stroke. 
Ling X, Zhang G, Xia Y, et al.
Journal of Cellular and Molecular Medicine, 2020

Exosomal transfer of p-STAT3 promotes acquired 5-FU resistance in colorectal cancer cells.
Zhang Q, Liu R X, Chan K W, et al.
Journal of Experimental & Clinical Cancer Research, 2019

Tumor-derived circulating exosomal miR-342-5p and miR-574-5p as promising diagnostic biomarkers for early-stage Lung Adenocarcinoma.
Han Z, Li Y, Zhang J, et al.
International Journal of Medical Sciences, 2020

A Panel of Plasma Exosomal miRNAs as Potential Biomarkers for Differential Diagnosis of Thyroid Nodules.
Liang M, Yu S, Tang S, et al.
Frontiers in Genetics, 2020

Embryonic Stem Cells-Derived Exosomes Endowed with Targeting Properties as Chemotherapeutics Delivery Vehicles for Glioblastoma Therapy.
Zhu Q, Ling X, and Deng Z et al.
Advanced Science, 2019

Renoprotective Effects of Artemisinin and Hydroxychloroquine Combination Therapy on IgA Nephropathy via Suppressing NF-κB Signaling and NLRP3 Inflammasome Activation by Exosomes in Rats.
Bai L, Li J, and Li H et al.
Biochemical Pharmacology, 2019

Urine-Derived Stem Cells Facilitate Endogenous Spermatogenesis Restoration of Busulfan-Induced Nonobstructive Azoospermic Mice by Paracrine Exosomes.
Deng C, Xie Y, and Zhang C et al.
Stem Cells and Development, 2019

Magnetic-Based Microfluidic Device for On-Chip Isolation and Detection of Tumor-Derived Exosomes.
Xu H, Liao C, Zuo P, Liu Z, and Ye B.
Analytical Chemistry, 2018

Multiple antibodies identify glypican-1 associated with exosomes from pancreatic cancer cells and serum from patients with pancreatic cancer.
Dong C, Huang L, Kalluri R. et al.
bioRixv, 2018

Serum Membrane Type 1-Matrix Metalloproteinase (MT1-MMP) mRNA Protected by Exosomes as a Potential Biomarker for Gastric Cancer.
Dong Z, Sun X, Xu J, et al.
International Medical Journal of Experimental and Clinical Research, 2019


Label‐Free Analysis of Single Viruses with a Resolution Comparable to That of Electron Microscopy and the Throughput of Flow Cytometry.
Ma L, Zhu S, Tian Y, et al.
Angewandte Chemie, 2016

Genetically Engineered Cell Membrane Nanovesicles for Oncolytic Adenovirus Delivery: A Versatile Platform for Cancer Virotherapy.
Lv P, Liu X, and Liu G et al.
Nano Letters, 2019

Nanomedicines (Liposomes)

Analytical techniques for single-liposome characterization.
Chen C, Zhu S, and Yan X et al.
Analytical Methods, 2013

Single-Particle Characterization of Theranostic Liposomes with Stimulus Sensing and Controlled Drug Release Properties. 
Chen C, Gao K, and Yan X, et al.
Biosensors and Bioelectronics, 2019


Detection and Quantification of Bacterial Autofluorescence at the Single-Cell Level by a Laboratory-Built High-Sensitivity Flow Cytometer.
Yang L, Zhou Y, and Yan X et al.
Analytical Chemistry, 2012

Sensitive and Selective Bacterial Detection Using Tetracysteine-Tagged Phages in Conjunction with Biarsenical Dye.
Wu L, Huang T, and Yan X et al.
Angewandte Chemie International Edition, 2011

Trace Detection of Specific Viable Bacteria by Using Tetracysteine-Tagged Bacteriophages.
Wu L, Luan T, and Yan X et al.
Analytical Chemistry, 2014

Rapid, Absolute, and Simultaneous Quantification of Specific Pathogenic Strain and Total Bacterial Cells Using and Ultrasensitive Dual-Color Flow Cytometer.
Yang L, Wu L, and Yan X et al.
Analytical Chemistry, 2010

Rapid quantification of pathogenic Salmonella Typhimurium and total bacteria in eggs by nano-flow cytometry.
C Mao, C Xue, X Wang, et al.
Talanta, 2020

Probing minority population of antibiotic-resistant bacteria.
Huang T, Zheng Y, Yan Y, et al.
Biosensors and Bioelectronics, 2016

Rapid quantification of live/dead lactic acid bacteria in probiotic products using high-sensitivity flow cytometry.
He S, Huang T, Zhang W, and Yan X et al.
Methods and Applications in Fluorescence, 2017


High-Throughput Multiparameter Analysis of Individual Mitochondria.
Zhang S, Zhu S, and Yan X et al.
Analytical Chemistry, 2012

Quantification of protein copy number in single mitochondria: The Bcl-2 family proteins.
Chen C, Zhang X, Zhang S, et al.
Biosensors & Bioelectronics, 2015

Identification of Mitochondria-Targeting Anticancer Compounds by an in Vitro Strategy.
Zhang X, Zhang S, and Yan X et al.
Analytical Chemistry, 2014

Rapid and quantitative in vitro analysis of mitochondrial fusion and its interplay with apoptosis.
Xu J, Su L, Han J, et al.
Talanta, 2021


Measuring particle size distribution and mass concentration of nanoplastics and microplastics: addressing some analytical challenges in the sub-micron size range.
Caputo F, Vogel R, Savage J, et al.
Journal of Colloid and Interface Science, 2021

Progress in the development of techniques based on light scattering for single nanoparticle detection.
Zhu S, Wang S, and Yan X et al.
Science China Chemistry, 2011

Light-Scattering Detection below the Level of Single Fluorescent Molecules for High-Resolution Characterization of Functional Nanoparticles.
Zhu S, Ma L, Wang S, et al.
ACS Nano, 2014

Development of an Ultrasensitive Dual-Channel Flow Cytometer for the Individual Analysis of Nanosized Particles and Biomolecules.
Yang L, Zhu S, and Yan X et al.
Analytical Chemistry, 2009

Size Differentiation and Absolute Quantification of Gold Nanoparticles via Single Particle Detection with a Laboratory-Built High-Sensitivity Flow Cytometer.
Zhu S, Yang L, and Yan X et al.
Journal of American Chemical Society, 2010

High-Throughput Single-Nanoparticle Analysis of Metal-Enhanced Fluorescence in Free Solution Using Ag@SiO2 Core-Shell Nanoparticles.
Yan Y, Meng L, and Yan X et al.
ACS Sensors, 2017

Flow Cytometric Analysis of Nanoscale Biological Particles and Organelles.
Lian H, He S, Chen C, and Yan X.
Annual Review of Analytical Chemistry, 2019

Light-Scattering Sizing of Single Submicron Particles by High-Sensitivity Flow Cytometry.
Zhang W, Tian Y, and Yan X et al.
Analytical Chemistry, 2018

Multiparameter Quantification of Liposomal Nanomedicines at the Single-Particle Level by High-Sensitivity Flow Cytometry.
Yan X, Chen C, Zhu S, Wang S, et al.
ACS Applied Materials and Interfaces, 2017

Nano-Flow Cytometry

Phosphoproteomics identify arachidonic-acid-regulated signal transduction pathways modulating macrophage functions with implications for ovarian cancer.
Dietze, R., Hammoud, M. K., Gomez-Serrano, et al.
Theranostics, 2021

Heparanase from triple‑negative breast cancer and platelets acts as an enhancer of metastasis.
Yang, W. J, Zhang, et al.
International Journal of Oncology, 2020

Electrochemical Deposition of Perylene-based Thin Films from Aqueous Solution and Studies of Visible Light-driven Oxidation of Alcohols.
Zhuang J. L Shen, Y M,  et al.
ACS Applied Energy Materials, 2020

Rapid and Quantitative Measurement of Single Quantum Dots in a Sheath Flow Cuvette.
Wang S, Li L, and Yan X et al.
Analytical Chemistry, 2017

Flow Cytometric Single-Cell Analysis Enables Quantitative in vivo Detection of Protein-Protein Interactions via Relative Reporter Protein Expression Measurement.
Wu L, Wang X, and Yan X et al.
Analytical Chemistry, 2017

High-throughput single-cell analysis of low copy number beta-galactosidase by a laboratory-built high-sensitivity flow cytometer.
Yang L, Huang T, and Yan X et al.
Biosensors & Bioelectronics, 2013


Preparation of therapeutic exosomes using membrane proteins.
Dooley K. P, Harrison, R A, et al.
U.S. Patent Application No. 16/722,884.

Compositions of engineered exosomes and methods of loading luminal exosomes pay-loads.
McConnell, Russell E., et al.
U.S. Patent Application No. 16/765,116.

Application of the Caveolin-1 protein positive excretion body as Diagnosis of Non-small Cell Lung marker.
Zheng Lei, Zhang Han, An Taixue, Situ Bo, Li Bo.
CN110095604A, 2019-08-06.