EV-inspired liposomes

Author: admin     Date: February 21, 2024

EVs can be genetically engineered to overexpress CD47, a protein that enables cargos to escape from clearance by the immune system and lead to enhanced accumulation. Liposomes are synethetic nanoparticles that capable of encapsulating thermosensitive drugs. The fusion of these two nanoparticles can exert the advantages of both exosomes and liposomes, with an effect of “1 + 1 > 2”. The resulting genetically engineered exosomes-thermosensitive liposomes hybrid nanoparticles (gETL NPs) bears CD47 proteins on the surface and thermosensitive drugs inside. The Flow NanoAnalyzer was employed to analyze the liposomes, exosomes and gETL NPs at single-particle level, which played important roles in the optimization and screening of conditions for genetically engineered exosomes and the production of gETL NPs.

                             Figure 1. Exosomes-Liposomes hybrid nanoparticles                    Figure 2. Detection of CD47 expression ratio in exosome-liposome nanoparticles

Adv. Sci., 2020, 7(18), 2000515.