Breast cancer

Author: admin     Date: February 21, 2024

Extracellular vesicle-associated miRNAs (EV-miRNAs) are emerging as a new type of noninvasive biomarker for disease diagnosis. Their relatively low abundance, however, makes accurate detection challenging. In this article, the authors designed a DNA nanowire guided-catalyzed hairpin assembly (NgCHA) nanoprobe for profiling EV-miRNAs. NgCHA showed high penetrability to EVs, which allowed rapid delivery of the probes into EVs. In the presence of targeted miRNAs within EVs, a fluorescent signal could be generated and amplified by confining the catalytic hairpin assembly system within the nanowires, thus greatly enhancing the analytical sensitivity. In the study, co-localisation analysis of NgCHA and EVs was performed using the Flow NanoAnalyzer to confirm that NgCHA enters the EVs lumen and generates a fluorescent signal. The platform is highly sensitive, cost-saving and simple to use compared to existing methods. Therefore, it has applications for noninvasive diagnosis of cancer.

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Figure 1. Colocalization analysis of NgCHA labeling positivity and EVs

The Flow NanoAnalyzer can achieve simultaneous detection of the scattering and fluorescence signal of the particles, i.e. co-localization analysis, with the advantage of high detection sensitivity and speed.

ACS Sensors, 2022, 7(4), 1075-1085.